Figure 3.

Contribution of both Gα_o and Gα_i to coupling between α₂-ARs and N-type Ca²⁺ channels with some degree of specificity. (A) Superimposed current traces in the absence or presence of 10 μM NE recorded from neurons coexpressing PTX-insensitive mutants (C351G) of different Gα subunits (Gα_oB and Gα_i1-i3) and Gβ₁γ₂. (B) Summary of VD Ca²⁺ current inhibition by NE. Data are presented as mean ± SEM, and numbers in parentheses indicate the number of neurons tested.