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Cytotoxic activities of 5-Helix-PE and C34-PE. Env+ (filled symbols) and Env− (open symbols) Chinese hamster ovary cells were incubated with 5-Helix (A), 5-Helix-PE (A), or C34-PE (B) in the absence or presence of sCD4 for 48 h. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide oxidation procedure and normalized to 100% for no added protein. The results are representative of three independent experiments.
