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. 2003 Apr 11;100(9):5040–5045. doi: 10.1073/pnas.0330962100

Figure 1.

Figure 1

Effects of SERCA1a and NF-SLN mutations on formation of the binary NF-SLN/SERCA1a complex. (A and B) SERCA1a mutants were expressed in HEK-293 cells in the presence or absence of NF-SLN. (C) wt SERCA1a was expressed in HEK-293 cells in the presence of wt or mutant NF-SLN. The cells were harvested after 48 h, and microsomal fractions were prepared and dissolved in Tween 20 as described (21, 26). NF-SLN was immunoprecipitated with the FLAG antibody, and the immunoprecipitate was separated by SDS/PAGE and stained with the A52 antibody against SERCA1a to determine the amount of SERCA1a coimmunoprecipitation. Numbers below each lane represent percentage of wt and are the average of three independent experiments.