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Effects of NF-SLN and the PLN I40A mutation on formation of the ternary PLN/NF-SLN/SERCA1a complex. SERCA1a was expressed in HEK-293 cells in the presence of NF-SLN and either wt or I40A mutant PLN. Microsomal fractions were dissolved and immunoprecipitated with antibody 1D11 against PLN. Immunoprecipitates were separated and stained with antibody A52 against SERCA1a. Numbers below lanes represent percentage of wt and are the average of three independent experiments.
