Skip to main content
. 2003 Apr 18;100(9):5075–5080. doi: 10.1073/pnas.0831227100

Table 1.

Kinetic data of Ras–RBD interactions

Reaction kdiss/s−1 kass/M−1⋅s−1 KD/nM
1. wtRBD + wtRas⋅mGppNHp 7.4 4.5  × 107 160
2. RBD(ch) + wtRas⋅mGppNHp 5.9 3.5  × 107 170
3. RBD/L91NBD + wtRas⋅GppNHp 11 1.8  × 107 610
4. RBD/L91NBD + Ras⋅GppNHp(ch) 11 2.3  × 107 480
5. wtRBD/L91W/H + wtRas⋅GppNHp* 14 3.6  × 107 380

Reactions 1 and 2, kinetics were monitored by using the mant fluorescence of mGppNHp. Reactions 3 and 4, kinetics monitored by using the NBD-Dap fluorescence. Reaction 5, kinetics monitored by using the tryptophan fluorescence from ref. 7. wt, wild type; H, His tag.