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High-molecular-weight ubiquitin conjugates formed in reticulocyte lysate after translation of RDTASIA γ2, RDTASIA γ2 + 50 μM MG132, or RDTASIA γ2 noK (all lysine residues substituted with arginine). Gels shown are from [35S]methionine-labeled reaction aliquots terminated 20 min after the start of translation.
