Figure 4.

Lysine residues required for the ubiquitin-dependent degradation of RDTASIA γ₂. (A) RDTASIA γ₂ (containing seven lysine residues), RDnoK (arginine substitution at all residues indicated), and various constructs containing lysine residues that were reintroducted by mutagenesis techniques. (B) [³⁵S]Methionine-labeled protein produced from RDTASIA γ₂ constructs depicted after translation in reticulocyte lysate, in the absence or presence of dipeptide inhibitors.