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. 2003 Apr 15;100(9):5103–5106. doi: 10.1073/pnas.0931345100

Figure 1.

Figure 1

(A) cDNA of mouse GRx. Sequence in bold corresponds to that of the dsRNA used in RNAi. (B) Construction of pBI-dsGRx. Sense and antisense DNA sequences corresponding to bases 11–300 of mouse GRx DNA sequences were inserted into MCS I and II regions, respectively. Primers for sense DNA contained MluI/NheI restriction sites: 5′-ggccacgcgtAgtttgtgaactgcaagatccagt-3′ and 5′-ccgggctagcCttcagccgagtcatcagctcccc-3′. Primers for antisense DNA contained NotI/SalI restriction sites: 5′-gacgcggccgcCttcagccgagtcatcagctcccc-3′ and 5′-ggccgtcgacAgtttgtgaactgcaagatccagt-3′.