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. 2003 Apr 15;100(9):5103–5106. doi: 10.1073/pnas.0931345100

Figure 4.

Figure 4

Lack of actin reorganization in GRx-knocked-out cells. NIH 3T3 cells stably expressing tetracycline-responsive reverse transactivator were cotransfected with YFP-actin and pBI-dsGRx and were grown on 25-mm-diameter circular glass plates coated with gelatin. Cells with or without GRx knockout (with or without doxycycline treatment, respectively) were starved in DMEM without serum for 3 h before treatment with FGF. Fluorescent images were monitored by using a Zeiss LSM 510 confocal microscope with a 488-nm laser and ×63 objective. Similar image changes were observed in >90% of the YFP-actin-transfected cells. (A) YFP-actin and pBI-dsGRx-transfected cells treated with doxycycline for GRx knockout before FGF treatment. B and C were recorded 20 and 380 s after FGF treatment, respectively. (D) YFP-actin and pB1-dsGRx-transfected cells before FGF treatment. E–I were recorded 20, 160, 220, 300, and 380 s after FGF treatment, respectively.