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PPase activity in nuclear extract. P3 peptide was used as substrate. (A) PPase activity in crude nuclear extracts (5 μg) from fasted and high-carbohydrate-fed rat livers was assayed as in Fig. 2. (B) Various sugar phosphates (50 μM) were added to the assay mixture and PPase activity was determined as in Fig. 2B. The same results were obtained with P4 peptide as a substrate (data not shown).
