Figure 3.

(A and B) Representative examples of the peripheral cytoplasm of BHK21-tTA/anti-CHC cells expressing CHC antisense RNA (without tet for 2 days) and fixed in the presence of ruthenium red. Note the ruthenium red-stained clathrin-coated profiles (arrowheads). One such profile is associated with a tubule (large arrow in A); several very small vesicular profiles, which may actually represent cross-sectioned tubules, are also shown (small arrows in A). (C–E) Ultracryosections of the cells expressing CHC antisense RNA (for 3 days). The sections are ImmunoGold-labeled (with Hudy 1 followed by 5-nm gold particles) to identify dynamin, which is present not only on clathrin-coated vesicular profiles (arrowheads) but also on tubular structures (arrows). (Bar = 200 nm.)