Figure 5.

Expression of dynamin is increased in the CHC antisense RNA cells. (A) Increased rate of dynamin synthesis. The cells were grown with or without tet for 2 days. Then they were pulse-labeled with [³⁵S]Met/[³⁵S]Cys (30 min) and solubilized. Dynamin was immunoprecipitated and resolved on a 10% SDS/PAGE gel. (B) Northern blot analysis of dynamin 2 mRNA. Total cellular RNA was isolated from cells grown with or without tet for 2 days, and 10 and 20 μg of each RNA sample, as indicated, were loaded onto a 1% agarose/formaldehyde gel and blotted onto a nylon membrane. Hybridization was carried out at 60°C with a labeled cDNA probe for dynamin 2. Band intensities on the blot were quantified with a PhosphorImager.