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. 2003 Apr 16;100(9):5187–5192. doi: 10.1073/pnas.0931282100

Figure 3.

Figure 3

PI 4-kinase activity is required for RRP refilling and insulin secretion in mouse β cells. (A) PI 4-kinase (open circles) and PI 5-kinase activities (filled circles) measured in islet homogenates in the presence of 0–50 μM PAO. (B) Insulin secretion measured during 1 h in the presence of 30 mM extracellular K+ and 0.2 mM diazoxide at 5 mM (open bar) and 20 mM glucose plus 0–50 μM PAO (filled bars). (C) Histogram summarizing exocytosis elicited by first (2 min, open bars) and second train of depolarizations (5 min after reaching whole-cell configuration, filled bars) under control conditions and in the presence of 20 μM PAO with or without 1 μM of PI(4)P and after inclusion of antibody directed against type II PI 4-kinase (anti-4C5G). Data are presented as mean values ± SEM of four (A), five (B), and five (C) experiments. *, P < 0.01.