Enhancer-blocking activity of scs and SF1b insulators paired with homologous and heterologous insulators. (A, B, and D–F) LacZ expression in transgenic embryos containing double insulators between the PE and E3 enhancers. Enhancement in the block of the PE enhancer is seen with PCC3 (A), PCB3 (B), PSF1bC3 (D), PSF1bSF1b3 (E), and PSF1bB3 (F). (C) LacZ expression in a PSF1b3 transgenic embryo.