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Enhancer-blocking activity of insulators pair with the MAR element. (A) LacZ expression in a PM3 transgenic embryo, showing a composite pattern directed by three enhancers. (B–D) LacZ expression in transgenic embryos containing double insulators. Enhancement in the block of PE is seen with PSM3 (B) and PCM3 (C) but not in PSF1bM3 (D; also see Table 1).
