Figure 2.
bir-1 RNAi does not influence the expression of CeSKIP∷gfp. (A) An animal expressing the CeSKIP∷gfp transgene after treatment with bir-1 RNAi. The expression of CeSKIP is not decreased by bir-1 RNAi. The strong expression is in pharynx (arrowhead), neurons, epidermal cells (arrows) in head region, and in ventral neuronal cord. (B) Distal part of the same animal showing expression of CeSKIP∷gfp in neurons, epidermal cells, and intestinal muscles. Note the properly developed intestinal muscles expressing CeSKIP∷gfp (arrow). (C) The presence of Egl phenotype and multinucleate embryos in the same animal as shown in A documenting the developmental defects caused by bir-1 RNAi (Nomarski optics). The arrow indicates the position of the egg-laying organ shown in D. (D) The strong expression of CeSKIP∷gfp in ventral neuronal cord (arrows) and in egg-laying muscles (arrowhead), which are also normally developed.