Table 4.
Translation repression in nus and rpoC mutants
| Mutation | nun
|
% expression | |
|---|---|---|---|
| − | K106/107D | ||
| Experiment I | |||
| None | 535 | 27 | 5 |
| nusA E132K | 456 | 85 | 19 |
| rpoC D329G | 1597 | 488 | 31 |
| rpoC D264G | 1485 | 256 | 17 |
| rpoC R322H | 1089 | 227 | 21 |
| Experiment II | |||
| None | 444 | 8 | 2 |
| nusBΔ | 992 | 17 | 2 |
Strains in Experiment I, rIIIΔ; Experiment II, rnc14∷Tn10. Induction is for 1 hr at 42°C. Values indicate β-galactosidase activity in Miller units (22). NusBΔ strains are cs. In Experiment II, therefore, strains were grown to mid-log at 37°C prior to shifting to 42°C for 1 hr.