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. 2003 Apr 15;100(9):5336–5341. doi: 10.1073/pnas.0931259100

Figure 4.

Figure 4

PD-L1 and PD-L2 up-regulation are regulated by Stat1 and Stat6. (A) PD-L1 up-regulation by LPS and IFN-γ depends on Stat1. Thioglycolate-induced macrophages from BALB/c, Stat1−/−, and C3H/HeJ mice were cultured in medium alone (control) or activated for 24 h with LPS (10–200 ng/ml) and IFN-γ (10–200 ng/ml) or LPS and IFN-γ together (10–200 ng/ml). Representative histograms shown are for activation at 100 ng/ml. (B) PD-L2 induction depends on IL-4Rα signaling and the Stat6 pathway. Thioglycolate-induced macrophages from BALB/c, Stat6−/−, and IL-4Rα−/− mice were activated for 24 h as described before and stained for PD-L1 and PD-L2 expression. Data shown are representative of at least three experiments with both Stat6−/− and IL-4Rα−/− mice. (C) Time course of PD-L1 and PD-L2 up-regulation on IL-4Rα- and Stat6-deficient macrophages. Macrophages were activated and harvested as described before and stained together at the end of the experiment. Time course data are representative of two experiments.