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. 2000 Jan 18;97(2):942–947. doi: 10.1073/pnas.97.2.942

Figure 2.

Figure 2

Gain-of-function 35S::ANT transgene expression causes multiple organ hyperplasia. (A–H) Arabidopsis. (I and J) tobacco. A, C, and E–G show plants or organs of 35S-vector-only control (Left or Upper) and 35SANT (Right or Lower) transformants grown under the long-day conditions. Specimens in each panel were photographed together (A, C, G, and H), or are shown at the same magnification (E and F). (A) Whole plants after bolting. (B) Leaf mass. The average from four independent 35S::ANT and control transgenic plants is shown. Bars indicate SD. (C) Mature flowers. (D) Mass of 10 flowers each from four independent 35S::ANT (AYM 18, 19) and control (AYM 27) T1 transgenic plants. (E) Mature anthers. The 35S::ANT anther fails to dehisce. (F) An enlarged ovule from the weak 35S::ANT transformant AYM19.4.17 with abnormal growth of nucellus cells. Embryo sac (asterisk) and nucellus cells (arrowhead) are indicated. (G) A fruit obtained after hand-pollination of an AYM19.4.17 pistil with wild-type pollen and an autonomously self-pollinated control fruit. (H) ant-1 (left), control (middle), and 35S::ANT (right) plants grown under short-day (8-h light) conditions. (I) KmR R1 tobacco seeds obtained from hand-pollination. (J) Two-day-old tobacco seedlings.