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A Michael-type addition reaction between vinyl sulfone-functionalized multiarm PEGs and mono-cysteine adhesion peptides (step 1, in high stoichiometric deficit) or bis-cysteine MMP substrate peptides (step 2, to come up to stoichiometric equivalence) was used to form gels from aqueous solutions in the presence of cells. These elastic networks were designed to locally respond to local protease activity at the cell surface (step 3).
