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Kinetic analysis of MMP-1 degradation of two oligopeptides, GCRD-GPQG↓IAGQ-DRCG (●) and GCRD-GPQG↓IWGQ-DRCG (□), bearing MMP substrates flanked by charged amino acids and a Cys on both sides. Degradation was followed fluorometrically by the reaction of the free amine group with fluorescamine. Lineweaver–Burk plots yielded the corresponding kinetic parameters kcat and Km, for the enzymatic reaction.
