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Synthetic LXR ligand promotes glucose uptake in 3T3-L1 adipocytes. (A and B) 3T3-L1 adipocytes at day 10 of differentiation were incubated for 24 h with serum-free medium supplemented with T1317 and/or insulin as indicated. The following day, glucose uptake of treated cells was measured in 96-well Cytostar-T microplates using 14C-labeled 2-deoxyglucose. (C) LXR agonist increases GLUT4 mRNA expression in 3T3-L1 cells. Gene expression was determined by real-time quantitative-PCR assays.
