Table 1.

Primer sequences and PCR parameters

Gene	Primers (5' → 3')	Temperature*
Rat eNOS	GCAAGACCGATTACACGACA GTCCTCAGGAGGTCTTGCAC	Denaturation Annealing Extension Signal	95°C-0 sec 57°C-5 sec 72°C-10 sec 85°C-1 sec
Human eNOS [42]	CCTCCAGGAAGGAGCAAAC TCCTGAGAGAGAGGCAAGAGGA	Denaturation Annealing Extension Signal	95°C-0 sec 55°C-5 sec 72°C-8 sec 81°C-1 sec
Rat iNOS	ATGGAACAGTATAAGCGAAACACC GTTTCCGGTCGATGTCATGAGCAAAGG	Denaturation Annealing Extension Signal	95°C-0sec 57°C-5 sec 72°C-10 sec 83°C-1 sec
Human iNOS [42]	CCCCATCAAGCCCTTTACTT CACCTCCTGGTGGTCACTT	Denaturation Annealing Extension Signal	95°C-0 sec 55°C-5 sec 72°C-8 sec 81°C-1 sec
Rat ET-1	GCTCCTGCTCCTCCTTGATG CTCGCTCTATGTAAGTCATGG	Denaturation Annealing Extension Signal	95°C-0 sec 55°C-5 sec 72°C-20 sec 84°C-1 sec
Human ET-1	AAGCCCTCCAGAGAGCGTTAT CCGAAGGTCTGTCACCAATGT 6FAM-TGACCCACAACCGAG-MGBNFQ	Denaturation Annealing Extension Signal	95°C-0 sec 55°C-5 sec 72°C-4 sec 72°C-1 sec
Human HO-1 [43]	TGATAGAAGAGGCCAAGA TTTCCAGACAGAGGGACA	Denaturation Annealing Extension Signal	95°C-0 sec 50°C-10 sec 72°C-17 sec 84°C-1 sec
Human HO-2 [43]	TGGAGCGCAACAAGGACCAT CCGGTAGAGCTGCTTGAACT	Denaturation Annealing Extension Signal	95°C-0 sec 50°C-10 sec 72°C-17 sec 84°C-1 sec
β-actin	CCTCTATGCCAACACAGTGC CATCGTACTCCTGCTTGCTG	Denaturation Annealing Extension Signal	95°C-0 sec 58°C-5 sec 72°C-8 sec 83°C-1 sec

* Initial denaturation was carried out at 95 °C for 3 minutes.