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. 2003 Apr 18;100(9):5443–5448. doi: 10.1073/pnas.0836639100

Figure 1.

Figure 1

Detection of pilin protein in wild-type and fibril− mutants by Western blotting analysis. (A) Whole-cell pilin. Lanes 1–4, whole-cell lysates from 5 × 107 DK1622, SW504, DSP1689, and SW301 cells, were separated by SDS/15% PAGE, electroblotted, and probed with a polyclonal antibody against PilA. The apparent molecular mass of PilA is ≈25 kDa. (B) Cell-surface pilin. Lanes 1–4, pilin was sheared off from 109 DK1622, SW504, DSP1689, and SW301 cells and was immunoblotted as in A. Shown are representative data for triplicate experiments.