Table 2.
Exp. No. (with MS5) | Control-CM | Wnt3A-CM | Inhibition Index* |
1 | 2182 ± 427 | 184 ± 91 | 0.08 |
2 | 9440 ± 1953 | 2652 ± 721 | 0.28 |
3 | 7292 ± 1928 | 2524 ± 475 | 0.35 |
Exp. No. (with BMS) | Medium | rmWnt3A | Inhibition Index* |
1 | 1746 ± 300 | 920 ± 64 | 0.53 |
BM CD10+ cells were cultured on a layer of the murine stromal cell line MS-5 in the presence of Wnt3A-conditioned medium (Wnt3A-CM) or control-conditioned medium (control-CM), or on a layer of human bone marrow stromal cells (BMS) in the presence of rmWnt3A or control medium. The numbers in the table represent the mean of duplicate wells ± SD. *Number of CD19+ cells in wells containing Wnt3A divided by number of cells in wells containing control-medium.