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. 1999 Feb 16;96(4):1181–1186. doi: 10.1073/pnas.96.4.1181

Figure 2.

Figure 2

Rapid syntheses of PnIA(A10L), PR(81–99), and [Glu40Gly]-CP10(1–40)-αCOSR assembled in 67 min, 2.1 h, and 4.4 h, respectively. Peaks labeled with ∗ indicate residual p-cresol or resorcinol from the HF cleavage. (A) HPLC and ES-MS analysis of the crude PnIA(A10L) reaction products. The major HPLC peak has the correct mass for PnIA(A10L). The HPLC peak labeled with ♦ refers to the PnIA(A10L) peptide with an oxidized Cys(p-MeBzl) group. (B) HPLC and ES-MS analysis of the crude PR(81–99) synthesis. The HPLC peak labeled with ▿ indicates the des-Arg deletion product. In the deconvoluted ES-MS spectrum (Right) peaks A, B, C, D, E, and F correspond to the presence of the des-Arg, des-Gln, des-Asn/Ile/Leu, des-Thr, and des-Gly deletion side-products and the correct peptide with an oxidized Cys(p-MeBzl) group. (C) HPLC and ES-MS analysis of the crude [Glu40Gly]CP10(1–40)-αCOSR synthesis. The HPLC peak labeled with ○ indicates the des-Leu/Ile deletion side-product. In the deconvoluted ES-MS spectrum (Right) peaks A, B, and C correspond to a double des-Ile/Leu deletion, single des-Ile/Leu deletion, and [Glu40Gly]CP10(1–40)-αCOSR containing residual glutamyl O-cyclohexyl ester protection, respectively.