Figure 6.

Rapamycin blocks reelin-induced [³⁵S]methionine incorporation into proteins. (A) Rapamycin dose dependently inhibits reelin (30 pM) stimulation of [³⁵S]methionine incorporation into SNS proteins. Each value is the mean ± SE of triplicate assays. (Inset) Western immunoblot with mTOR-specific antibody. TB, total brain. (B) Western immunoblot of reelin bound to SNSs after incubation with or without reelin in the presence or absence of different doses of rapamycin. Bars represent immunofluorescence intensity ratio between reelin (r) and β-actin (a). Lane 1, non-treatment; lane 2, reelin; lanes 3–5, reelin plus rapamycin; lanes 6–8, only rapamycin.