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. 2003 Apr 11;100(9):5485–5490. doi: 10.1073/pnas.0830111100

Figure 4.

Figure 4

Repeated injections of ethanol led to ethanol tolerance that can be inhibited by pretreatment with 3-APMPA but not with CGP 54626. (A) Ethanol (EtOH; 800 nmol per fly) was injected twice (interval of 1–24 h), or flies were injected first with vehicle (Veh) and then with EtOH (nontolerant controls). The duration of immobility of flies treated with two EtOH is expressed as the percentage of immobility of the corresponding Veh + EtOH-treated controls (*, P ≤ 0.001; **, P ≤ 0.01; Student's t test; n = 7–16 flies per group and per time interval). The interval between the first injection (3-APMPA, 2 pmol per fly, or CGP 54626, 0.2 nmol per fly) and second injection (EtOH, 400 nmol per fly) (B and C) was 1 h, and the interval between the second and third injections was 4 h (to induce EtOH tolerance). All groups differed significantly from vehicle-only-injected flies; significant differences between EtOH-treated groups are indicated (*, P ≤ 0.05; **, P ≤ 0.01; Scheffé's test; n = 13).