Figure 3.

Identification of genes differentially expressed in the retina by statistical analysis. (a) Representative scatter plots of estimated contrast versus average intensity. The y axis shows the estimated contrasts between two regions of the retina and the x axis shows the average intensity values for all data. Two of six plots are shown [(NT) and (DV)]. Genes with average intensity >256 (2⁸) included for further analysis are red. Five genes validated by in situ hybridization (see Fig. 4) are highlighted: Id3 (green), μ-crystallin (purple), CRABP1 (aqua), e(y)2 (pink), and NEDF (blue). (b) Cluster dendrogram of the average expression profiles of the nine groups. (c) Visualization of the average expression profiles of the nine groups using a red/green diagram (41) where the order corresponds to the dendrogram in b. Red and green indicate increased or decreased relative expression, respectively. Black indicates equivalent expression, all relative to the average across all regions.