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. 2003 Apr 17;100(9):5491–5496. doi: 10.1073/pnas.0831080100

Figure 4.

Figure 4

In situ hybridization of predicted differentially expressed genes. (a–c) Id3. Dark-field view of horizontal section (a) or coronal section (b) of E14.5 eye hybridized in situ with Id3 probe. Note signal in the ventricular zone in a high-T to low-N and high-D to low-V gradient (compare areas marked by asterisks in a and b). A four-way expression prediction (in silico average) for Id3 is shown in c. (d–f) μ-Crystallin. μ-Crystallin probe labels D-T retina (asterisks in d and e) and the optic disk (arrows in d and e). A four-way prediction for μ-crystallin is shown in f. (g–i) CRABP1. A CRABP1 probe labels the RGC layer in a high-V to low-D gradient (compare areas marked by arrowheads in h). Note equal signal in N and T (compare areas marked by arrowheads in g). A four-way prediction for CRABP1 is shown in i. (j–l) e(y)2. e(y)2 probe labels the RGC layer in a high-D to low-V gradient (compare areas marked by arrowheads in k). A four-way prediction for e(y)2 is shown in l. (m–o) NEDF. NEDF is expressed in the RGC layer in a high-D to low-V gradient (compare areas marked by arrowheads in n). A four-way prediction for NEDF is shown in o. (Scale bar, 100 μm.)