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Effects of altered VirB6 synthesis on T-pilus production and virulence at 20 and 28°C. (A) Exocellular VirB2 pilin content as a measure of T-pilus production of strains grown at 20 and 28°C. A348, wild-type; ΔB6(PvirB-B6), PC1006(pSJB964); ΔB6(PlacZ-B6), PC1006(pXZB61); ΔB6 (PC1006) elaborates no T pilus (21, 30, 39). (B) Virulence of corresponding strains on K. daigremontiana leaves maintained at the temperatures shown.
