FIG. 1.

DNA sequence of the codBA promoter region showing key features. The DNA sequence is as found in pRAC82. Small letters represent vector DNA; capital letters represent E. coli DNA. Boxed nucleotides are the NAC consensus sequence. Over- and underscoring show the extent of the DNase I footprint of NAC on the top and bottom strands of this fragment (Fig. 3 and data not shown). The arrowhead indicates the strong hypersensitive site. The letters with a line through them represent the segment that was deleted (replaced) in the shorter promoter in pCB910 and pCB911. The bold letters at position +1 are the start sites of transcription (8). Underlined sequences are putative −10 and −35 regions, and GTG is the start site of the codB-coding sequence. The C above the A at position −66 indicates the nucleotide changed in the construction of pCB911 from pCB910.