Skip to main content
. 2003 May;185(9):2961–2966. doi: 10.1128/JB.185.9.2961-2966.2003

FIG. 2.

FIG. 2.

ScaI restriction maps of the clpX regions of genomic DNA of S. pneumoniae strains EL59, EL873, and EL1392 amplified by PCR with the oligonucleotide primer pair clpX-a and clpX-c (22). Lanes 1 and 7, Gibco 1-kb Plus standard ladder; lane 2, wild-type clpX+ locus from parent strain EL59; lane 3, duplicated spr1426 locus from EL1392, which contains a copy of clpX+ and the clpX<>ermAM cassette; lane 4, Erms revertant of EL1392 containing the wild-type clpX+ region; lane 5, clpX::ermAM with duplicated clpX+ locus from EL873; lane 6, Erms revertant of EL873 containing the wild-type clpX+ region. Shaded arrows, clpX reading frame; hatched arrows, ermAM reading frame; open arrows, genes surrounding clpX (Fig. 1); black dots, ∼0.9 kb of unknown DNA present in EL1392 clpX+ region. ScaI restriction sites are indicated with the letter S and a horizontal line. The break denotes an unknown amount of chromosomal DNA in the EL873 merodiploid. Predicted restriction fragment sizes and indicated DNA standards are in kilobase pairs.