TABLE 1.
Bacterial strainsa
| Strain | Genotype or phenotype | Description | Source or reference |
|---|---|---|---|
| EL59 | S. pneumoniae R6, avirulent unen- capsulated parent strain | Derived from D39 isolate | A. Tomasz (14) |
| EL161 | S. pneumoniae D39, virulent encap- sulated type 2 parent strain | Subclone of original clinical isolate | J. Yother (25) |
| EL539 | EL59 ΔclpP::aad9 (Spcr) | EL59 transformed with linear ΔclpP::aad9 amplicon | 22 |
| EL854 | EL59 ΔclpC::ermAM (Ermr) | EL59 transformed with linear ΔclpC::ermAM amplicon | 22 |
| EL873 | EL59 ΔclpX::ermAM clpX+ (Ermr) | EL59 containing previously undetected merodiploid of ΔclpX::ermAM and clpX+ | 22 |
| EL1039 | EL161 ΔclpX::ermAM clpX+ (Ermr) | EL161 transformed with genomic DNA from EL873; contains a previously undetected merodiploid of ΔclpX::ermAM and clpX+ | 22 |
| EL1082 | EL59 ΔclpL::ermAM (Ermr) | EL59 transformed with linear ΔclpL::ermAM amplicon | This study |
| EL1259 | EL59 ΔclpE::aad9 (Spcr) | EL59 transformed with linear ΔclpE::aad9 amplicon | This study |
| EL1383 | EL59 ΔbgaA::(Pc::tetL::T1T2::PfcsK-clpX+) (Tetr) | EL59 transformed with linear ΔbgaA::(Pc::tetL::T1T2::PfcsK-clpX+) amplicon | This study |
| EL1387 | EL1383 ΔclpX<>ermAM (Ermr Tetr) | EL1383 transformed with linear ΔclpX<>ermAM amplicon | This study |
| EL1392 | ΔclpX<>ermAM clpX+ (Ermr) | EL59 transformed with ΔclpX<>ermAM, resulting in a partially characterized duplication of ΔclpX<>ermAM and clpX+ | This study |
a
Strains were constructed by transformation of indicated recipients with linear double-stranded synthetic PCR amplicon DNA. Following single colony isolation, all constructions were confirmed by PCR analysis of chromosomal DNA by using flanking and internal primers and additional restriction digestion of amplicons (details available at http://www.streppneumoniae.com).