FIG. 2.
Northern blot analysis of icaADBC in static cultures of S. epidermidis in TA in the presence or absence of glucose. A 6-h preculture of S. epidermidis 1457 was diluted 1:100 in fresh TA (−) and in TA+glc (+) and cultivated in tissue culture plates at 37°C. After 12, 22, and 36 h of incubation, total bacterial RNA was extracted. Hybridization was performed with an icaC-specific oligonucleotide. In parallel, a biofilm assay was performed demonstrating a biofilm-positive phenotype in TA+glc. OD570s of 1.6, 1.7, and 1.8 were detected at 12, 22, and 36 h, respectively. In TA, S. epidermidis 1457 exhibited a biofilm-negative phenotype.