FIG. 2.

Binding of MBP-PerA to bfpA and perA. EMSAs with a bfpA fragment from positions −214 to −15 (A) and a perA fragment from positions −155 to +21 (B) were carried out with 0, 0.11, 0.21, 0.43, 0.87, 2.16, and 4.3 μg (0, 74 nM, 148 nM, 298 nM, 596 nM, 1.5 μM, and 3 μM, respectively) of purified MBP-PerA (lanes 1 to 7). Samples were resolved in 2.5% agarose gels, stained with ethidium bromide, and photographed with an Alpha-Imager.