FIG. 3.

Mapping of the PerA binding site on the bfpA regulatory region by EMSA. (A) Overlapping 200-bp-long PCR fragments that span the bfpA upstream and downstream promoter region from positions −374 to +262 were incubated with (+) or without (−) 2.16 μg (1.5 μM) of purified MBP-PerA. Samples were resolved in 6% native polyacrylamide gels and visualized by ethidium bromide staining. The asterisk denotes the second complex formed with fragment 7. (B) Schematic representation of the PCR fragments spanning the bfpA regulatory and structural regions. The fragment number is indicated to the left, and the sequence range covered for each fragment is indicated to the right. The approximate locations of the putative PerA binding sites, upstream and downstream of the bfpA promoter, are indicated by black and gray boxes, respectively. PBS, previously proposed PerA binding sequence