FIG. 5.
PerA binds to double-stranded oligonucleotides containing the putative PerA binding site. EMSAs were performed by incubating 32P-labeled double-stranded oligonucleotides of different lengths containing bfpA (A) or perA (B) sequences (as described in Tables 2 and 3, respectively), with 1.06 and 2.1 μM MBP-PerA. Control reactions with DNA alone were included as controls. A nonspecific 47-mer oligonucleotide containing an unrelated sequence was included as a negative control. In addition, control reactions with 50- and 100-fold molar excesses of the unlabeled 45-mer (PBSA45) were included to demonstrate the specificity of the interaction (data not shown). Samples were subjected to electrophoresis on a 6% native polyacrylamide gel.