TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains E2348/69 | Wild-type EPEC O127:H26, Nalr | J. B. Kaper |
| E2348/69 perA::Km | E2348/69 with a nonpolar insertional mutation in perA | Unpublished results |
| JPN15 | EAF plasmid-cured derivative of E2348/69, Nalr | J. B. Kaper |
| MC4100 | F−araD139 (argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbcR | Laboratory strain |
| BL21/pLys21 | F−ompT (lon) hsdSB(rB mB) gal dcm (λDE3) | Invitrogen |
| KY2263 | MC4100 clpPX-lon::Cm | 51 |
| KY2266 | MC4100 clpPX-lon::Cm hslVU::Tc | 51 |
| BB2395 | MC4100 lon146::mini-Tn10 Tcr | 51 |
| SU101 | E. coli JL 1434 lexA71::Tn5 (Def) sulA211 Δ(lacIPOZYA)169/F′ lacIqlacZΔM15::Tn9 (op+/op+) | 8 |
| Plasmids | ||
| pCST | pACYC184 derivative carrying perA (bfpT) | 23 |
| pMalC2xa | Vector for constructing MBP fusions | New England Biolabs |
| pMALT2 | pMalC2xa derivative expressing MBP-PerA | This work |
| pKK232-8 | pBR322 derivative containing a promoterless cat gene | Pharmacia Biotech |
| pCAT201 | pKK232-8 derivative containing the bfpA-cat transcriptional fusion from nucleotides −201 to +76 | 5 |
| pCAT201 + 27 | pKK232-8 derivative containing the bfpA-cat transcriptional fusion from nucleotides −201 to +27 | Unpublished results |
| pSR660 | Vector encoding the LexA DBD sequence used for homodimerization studies; TcroriV | 7 |
| pMI660-PerA | pSR660 derivative carrying the lexADBD-perA fusion | This work |
| pRS658-CAT | pSR660 derivative carrying the lexADBD-cat fusion | 7 |
| pRS658-HNS | pSR660 derivative carrying the lexADBD-hns fusion | Unpublished results |
| pMA660-AraC | pSR660 derivative carrying the lexADBD-araC fusion | This work |