TABLE 2.
Probe | Sequence relative to bfpA promoter region | Relative shift (%)b |
---|---|---|
−98−90−85−77−64−54−46−40−35 |||||||| | ||
GTACTGGGGGGGACGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTTTTCTTGGTGCTTGCGTGTCTT | ||
PBSA56 | GGGGACGGAAATATATAAAAAAAAAGAAAAAAAGATTATTTTTTTTCTTGGTGCT | 100 |
PBSA45 | GGGGACGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 100 |
PBSA35 | GGGGACGGAAATATATAAAAAAAAAAGAAAAAAAG | 19 |
PBSA25 | GGGGACGGAAATATATAAAAAAAAA | <1 |
PBSA2/2 | AGAAAAAAAGATTATTTTTTTTCTTGG | 0 |
PBSA39 | GGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 100 |
PBSA35-2 | ATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 47 |
Mut1 | GGGGACAACCCGATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 70 |
Mut2 | GGGGACGGAAATATCGCCCCAAAAAAGAAAAAAAGATTATTTTTT | 82 |
Mut3 | GGGGACGGAAATATATAAAAAAAAAAGAAAGGCCAATTATTTTTT | 43 |
Mut1-2 | GGGGACAACCCGATCGCCCCAAAAAAGAAAAAAAGATTATTTTTT | 21 |
Mut1-2-3 | GGGGACAACCCGATCGCCCCAAAAAAGAAAGGCCAATTATTTTTT | <1 |
5′Mut | TCTTCTGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 75 |
PBSAmG64 | GGGGACGGAAATATATAAAAAAAAAAAAAAAAAAGATTATTTTTT | 100 |
PBSAmG84 | GGGGACAGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT | 100 |
PBSA2-56c | TTATGGTTTCTAAAATCATGAATAAGAAATACGAAAAAGGTCTGTCTTTGATTGAA | 53d |
NE34e | TCGAGCTCTCTTCTGTTCTGCTTGACTTCCTAGG | <1 |
NE47e | GATCAGTCCTTGAATGGTGTGAAGTAAAAGTGCCTTCAAAGAATCCC | 4 |
Modified sequences are underlined. The boldface letters indicate the location of the minimal sequence required for PerA binding.
Estimated by EMSA relative to PBSA45.
Sequence corresponding to positions +38 to +93 of bfpA.
Relative to PBSA56.
Random sequences.