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. 2003 May;185(9):2835–2847. doi: 10.1128/JB.185.9.2835-2847.2003

TABLE 2.

Binding to double-stranded oligonucleotides corresponding to the bfpA regulatory regiona

Probe Sequence relative to bfpA promoter region Relative shift (%)b
−98−90−85−77−64−54−46−40−35 ||||||||
GTACTGGGGGGGACGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTTTTCTTGGTGCTTGCGTGTCTT
PBSA56 GGGGACGGAAATATATAAAAAAAAAGAAAAAAAGATTATTTTTTTTCTTGGTGCT 100
PBSA45 GGGGACGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 100
PBSA35             GGGGACGGAAATATATAAAAAAAAAAGAAAAAAAG 19
PBSA25             GGGGACGGAAATATATAAAAAAAAA <1
PBSA2/2             AGAAAAAAAGATTATTTTTTTTCTTGG 0
PBSA39             GGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 100
PBSA35-2             ATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 47
Mut1             GGGGACAACCCGATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 70
Mut2             GGGGACGGAAATATCGCCCCAAAAAAGAAAAAAAGATTATTTTTT 82
Mut3             GGGGACGGAAATATATAAAAAAAAAAGAAAGGCCAATTATTTTTT 43
Mut1-2             GGGGACAACCCGATCGCCCCAAAAAAGAAAAAAAGATTATTTTTT 21
Mut1-2-3             GGGGACAACCCGATCGCCCCAAAAAAGAAAGGCCAATTATTTTTT <1
5′Mut             TCTTCTGGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 75
PBSAmG64             GGGGACGGAAATATATAAAAAAAAAAAAAAAAAAGATTATTTTTT 100
PBSAmG84             GGGGACAGAAATATATAAAAAAAAAAGAAAAAAAGATTATTTTTT 100
PBSA2-56c             TTATGGTTTCTAAAATCATGAATAAGAAATACGAAAAAGGTCTGTCTTTGATTGAA 53d
NE34e             TCGAGCTCTCTTCTGTTCTGCTTGACTTCCTAGG <1
NE47e             GATCAGTCCTTGAATGGTGTGAAGTAAAAGTGCCTTCAAAGAATCCC 4
a

Modified sequences are underlined. The boldface letters indicate the location of the minimal sequence required for PerA binding.

b

Estimated by EMSA relative to PBSA45.

c

Sequence corresponding to positions +38 to +93 of bfpA.

d

Relative to PBSA56.

e

Random sequences.