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. 2003 May;185(9):2835–2847. doi: 10.1128/JB.185.9.2835-2847.2003

TABLE 3.

Relative binding to double-stranded oligonucleotides corresponding to the perA regulatory regiona

Probe Sequence relative to perA promoter region Relative shift (%)b
−81−75−66−54−40−35 |||||
CAACACCTTGAAAAATATCAGTAAATTTTTAAAAAAAAAGCATAAAAGTATTGATTATTTACAGAG
PBST56 CAACACCTTGAAAAATATCAGTAAATTTTTAAAAAAAAAGCATAAAAGTATTGATT 100
PBST45 CAACACCTTGAAAAATATCAGTAAATTTTTAAAAAAAAAGCATAA 100
PBST35 CAACACCTTGAAAAATATCAGTAAATTTTTAAAAA 8
PBST40-1 CCTTGAAAAATATCAGTAAATTTTTAAAAAAAAAGCATAA 100
PBST35-2 AAAAATATCAGTAAATTTTTAAAAAAAAAGCATAA 50
PBST40-2 GAAAAATATCAGTAAATTTTTAAAAAAAAAGCATAAAAGT 100
PBST35-3 GTAAATTTTTAAAAAAAAAGCATAAAAGTATTGAT 0
a

Modified sequences are underlined. The boldface letters indicate the location of the minimal sequence required for PerA binding.

b

Estimated by EMSA relative to PBST45.