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. 2003 May;185(9):2820–2825. doi: 10.1128/JB.185.9.2820-2825.2003

FIG. 2.

FIG. 2.

FIG. 2.

PlcR directly activates inhA2 transcription. Sequence of the inhA2 promoter region (A). The putative −10 and −35 boxes of the inhA2 promoter are boxed. The potential ribosome-binding site and the start codon (ATG) are underlined. The potential reverse Spo0A box and the PlcR box are shaded. The broken arrow indicates the transcription start site. Arrows indicate the positions of primers used in the DNase I footprinting experiments. DNase I footprinting analysis of PlcR binding to the inhA2 promoter region (B). Lane 1 indicates the A+G Maxam and Gilbert reactions of the labeled template strand of inhA2 promoter region. Lane 2, no PlcR; lane 3, 100 pmol of purified PlcR. Brackets indicate the region protected by PlcR.