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Two-dimensional gel electrophoresis of B. thuringiensis culture supernatants collected 2 h after the onset of the stationary phase. The gel area shown is the InhA/InhA2 zone, between isoelectric points 5.1 (left) and 5.9 (right) and in the molecular mass range from 70 to 90 kDa. (A) Strain 407 Cry−; (B) strain 407 Cry− ΔplcR; (C) strain 407 Cry− [ΔplcR (pinhA2)]; (D) strain 407 Cry− [ΔinhA2 (pinhA2)]. ColB, collagenase B; Ypr5, enhancin; NheB, component B of enterotoxin Nhe.
