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pH values measured after redissolution of lyophilized subtilisin in pure water
| Original pH 5.0 | Original pH 8.5 | |
|---|---|---|
| Na-phosphate | 5.6 | 8.4 |
| NaOOCH | 7.1 | 8.0 |
| NH4-phosphate | 3.9 | 3.9 |
| NH4OOCH | 4.8 | 4.5 |
Subtilisin was lyophilized from buffers based on 2 mM solutions of the salts shown. Samples were redissolved at 1 mg/ml in purified water, which had a measured pH of 5.35.
