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Detection of native Rsp proteins by Western blotting. Alkaline-extracted surface antigens and crude supernatant separated on SDS-PAGE were blotted onto polyvinylidene difluoride membranes and probed with anti-rRspA IgG (A) or anti-rRspB IgG (B). Lanes: 1, alkaline-extracted antigens of Fujisawa-SmR; 2, culture supernatant of Fujisawa-SmR; 3, alkaline-extracted antigens of SE-9; 4, culture supernatant of SE-9.
