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SDS-PAGE of rRsp proteins. rRspA and rRspB were expressed as His-tagged fusion proteins and purified on a nickel-nitrilotriacetic acid resin. The gel regions (arrowheads) containing either RspA or RspB were excised from the gels and used for immunization of rabbits. Lanes: 1, RspA; 2, RspB. Molecular size markers (in kilodaltons) are shown on the left.
