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Static biofilm formation assay. The abilities of PAO1 and its isogenic mutants to form biofilms in a static assay were compared. The amount of planktonic growth in each well as measured by the turbidity (reported here as optical density [O.D.]) at 600 nm is shown in white bars, while the amount of biofilm is shown as the absorbance of bound CV at 600 nm in black bars. Pilin (pilA) and flagellin (fliC) mutants were used as negative controls.
