FIG. 5.

Biofilm morphology of mutants. (A) SEM of P. aeruginosa grown in silicone tubing. Each individual strain was grown under flow in DMM+ media for 24 h (longer growth times resulted in thicker biofilms that collapsed during dehydration, making structure difficult to see). Magnification, 1,000×; bars, 10 μm. (B) CSLM of BacLight live/dead stained 48-h biofilms. Sagittal (x-z) sections through the biofilm are indicated by white arrows. Note that defects in biofilm formation are consistent despite differences in imaging techniques (SEM versus CSLM), substrata (silicone versus glass), and length of incubation (24 versus 48 h), showing that these defects are not specific to cultivation conditions. Magnification, 100×; bar, 100 μm.