TABLE 2.
Putative biofilm-induced proteins and their homologues
IVET clone ORF (no. of nucleotides) | Size (aa) | Selected homologuesa | Function | % Iden- tityc | % Simi- larityd | GenBank accession no. |
---|---|---|---|---|---|---|
9.27A PA0240 (1266) | 421 | PhaK-like protein, P. putida | Putative porin | 71 | 82 | AF031417 |
Pflu_p_1331, Pseudomonas fluorescens | Hypothetical protein | 69 | 81 | AABA01000097 | ||
Psyr0433, Pseudomonas syringae pv. syringae B728a | Hypothetical protein | AABH01000001 | ||||
BenF, P. putida | Benzoate-specific porin-like protein | 42 | 61 | AF218267 | ||
9.29 PA2247 (1233) | 410 | BkdA1, P. putida | 2-Oxoisovalerate dehydrogenase (alpha subunit) | 79 | 88 | AAA65614 |
BkdA1, Mesorhizobium loti | 2-Oxoisovalerate dehydrogenase (alpha subunit) | 64 | 76 | AP003004 | ||
9.27B and C PA3710 (1674) | 557 | Pflu_p_4963, P. fluorescens | Hypothetical protein | 76 | 85 | AABA01000160 |
SMc03116, Sinorhizobium meliloti | Putative alcohol dehydrogenase protein | 63 | 76 | AL591792 | ||
CC1278, C. crescentus | Oxidoreductase, GMC family | 55 | 68 | AE005802 | ||
AlkJ, P. putida | Aliphatic alcohol dehydrogenase | 40 | 57 | AJ233397 | ||
3.15 PA3782 (954) | 317 | Z0442, E. coli O157:H7 | Transcriptional regulator | 48 | 67 | CAB87229 |
mlr2766, M. loti | Transcriptional regulator | 49 | 63 | BAB49814 | ||
AdpA, S. griseus | AraC-like transcriptional regulator; streptomycin and aerial mycelium production | 48 | 62 | BAA86265 | ||
AdpA, S. coelicolorb | AraC-like transcriptional regulator | 47 | 62 | CAB87229 | ||
9.27D and E PA5065 (1602) | 542 | UbiB, E. coli | Ubiquinone biosynthesis | 56 | 70 | D65188 |
UbiB, Vibrio choleraeb | Ubiquinone biosynthesis | 55 | 69 | AAF93263 | ||
UbiB (AarF), Providencia stuartii | Ubiquinone biosynthesis | 59 | 74 | AAB96577 | ||
UbiB, Neisseria meningitidis | Ubiquinone biosynthesis | 57 | 72 | AAF40987 |
Homologous proteins, identity, and similarity scores were determined by the BLASTp program at NCBI.
Hypothetical protein.
Identical amino acids.
Identical plus conserved amino acids.