Fig. 1.
Kinetics of glucocorticoid-induced DNA demethylation at the −2.5 Tat GRU in hepatoma cells. (A) The four MeCpGs within the Tat GRU (−2,425 to −2,336) that are demethylated upon glucocorticoid treatment are numbered from 1 to 4 (upper strand, U1 to U4; lower strand, L1 to L4). (B) Demethylation kinetics of the three neighboring Cs of the upper strand. Rat hepatoma cells were grown with 10−7 M dexamethasone (+Dex) for the indicated time. The corresponding genomic DNA was treated with hydrazine and piperidine and analyzed by LM-PCR (refs. 19 and 40; Fig. 7). Genomic DNA cleaved at A+G and C+T was analyzed simultaneously.